FF421/491/572-Di01-25×36

Theoretical Spectrum for Part Number: FF421/491/572-Di01-25×36

421/491/572 nm BrightLine 三边缘标准落射荧光二向色镜，25.2 mm x 35.6 mm，厚度 1.1 mm

Semrock多边缘二向色镜具有从高反射率到高透过率的两个或多个转变。Semrock提供多种二向色镜分束器，具有陡峭的边缘和非常高的反射和透射率。这种标准落射荧光二向色镜分束器仅针对LED或类似光引擎等宽带光源进行了优化。
BrightLine二向色分束器，用于DAPI/GFP/mCherry成像通道，针对Lumencor CELESTA光引擎进行了优化，使用408 nm、473 nm、545 nm波长。

滤光片参数：

Optical Specifications	Value
Transmission Band 1	Tavg > 93% 426 – 456 nm
Transmission Band 2	Tavg > 93% 498 – 524 nm
Transmission Band 3	Tavg > 93% 584 – 680 nm
Transmission Band 4	Tavg > 93% 584 – 850 nm
Reflection Band 1	Ravg > 95% 350 – 400 nm
Reflection Band 2	Ravg > 95% 400 – 413 nm
Reflection Band 3	Ravg > 95% 471 – 483 nm
Reflection Band 4	Ravg > 95% 541 – 560 nm
Edge Wavelength 1	421 nm
Edge Wavelength 2	491 nm
Edge Wavelength 3	572 nm
General Filter Specifications	Value
Angle of Incidence	45 ± 1.5 degrees (Ravg > 95% & Tavg > 90%), 45 degrees (Tavg > 93%)
Cone Half-angle	2 degrees
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Reflected Wavefront Error	>> 6λ P-V RWE @ 632.8 nm
Steepness	Standard
Flatness / RWE Classification	Standard Epi-fluorescence
Physical Filter Specifications	Value
Transverse Dimensions (L x W)	25.2 mm x 35.6 mm
Transverse Tolerance	± 0.1 mm
Clear Aperture	≥ 80% (elliptical)
Scratch-Dig	60-40
Filter Thickness (unmounted)	1.05 mm
Filter Thickness (unmounted)	1.05 mm
Filter Thickness Tolerance (unmounted)	± 0.05 mm
Filter Thickness Tolerance (unmounted)	± 0.05 mm
Orientation	Reflective surface marked with part number – Orient in direction of incoming light