FF580-FDi01-25×36

Theoretical Spectrum for Part Number: FF580-FDi01-25×36

580 nm BrightLine 用于标准显微镜的荧光图像分离二向色镜，25.2 mm x 35.6 mm，厚度 1.1 mm

Semrock BrightLine图像分离二向色镜分束器在按颜色分离光束以同时捕获多色图像时，为透射光和反射光提供了理想的图像质量。对于（FRET）和实时活细胞成像等应用，用户现在可以将两种、四种甚至更多的颜色分离到尽可能多的相机或单个相机传感器的感光区域上。对于大多数常见的成像系统，这些滤光片的出色平坦度几乎消除了反射光束中的像差。这种二向色镜分束器经过优化，可以反射直径高达10毫米的成像光束，同时最大限度地减少反射波前差RWE。

常见的荧光团图像分离：GFP/mCherry 或 FITC/TxRed

如果您对改进的平面度Flatness/反射波前差RWE版本感兴趣，您也可以考虑Semrock用于超分辨率显微镜的图像分离二向色分束器：
3mm厚度 FF580-FDi02-t3上的反射波前差RWE为λ/5 P-V ，在定制尺寸版本中针对反射高达37mm的成像光束进行了优化。

滤光片参数：

Optical Specifications	Value
Transmission Band 1	Tavg > 93% 590.8 – 950 nm
Reflection Band 1	Ravg > 95% 350 – 570 nm
Edge Wavelength 1	580 nm
Common Fluorophore Pair to Split	GFP/mCherry or FITC/TxRed
General Filter Specifications	Value
Angle of Incidence	45 ± 1.5 degrees
Cone Half-angle	2 degrees
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Reflected Wavefront Error	< 2λ P-V RWE @ 632.8 nm
Steepness	Standard
Flatness / RWE Classification	Image-splitting
Physical Filter Specifications	Value
Transverse Dimensions (L x W)	25.2 mm x 35.6 mm
Transverse Tolerance	± 0.1 mm
Clear Aperture	≥ 80% (elliptical)
Scratch-Dig	60-40
Filter Thickness (unmounted)	1.05 mm
Filter Thickness (unmounted)	1.05 mm
Filter Thickness Tolerance (unmounted)	± 0.05 mm
Filter Thickness Tolerance (unmounted)	± 0.05 mm
Orientation	Reflective surface marked with part number – Orient in direction of incoming light