滤光片型号：FF421/491/567/659/776-Di01-25×36
FF421/491/567/659/776-Di01-25×36 五边缘标准落射荧光二向色镜透过率曲线，Semrock
421/491/567/659/776 nm BrightLine 五边缘标准落射荧光二向色镜，25.2 mm x 35.6 mm，厚度 1.1 mm
Semrock多边缘二向色镜具有从高反射率到高透过率的两个或多个转变。Semrock提供多种二向色镜分束器，具有陡峭的边缘和非常高的反射和透射率。这种标准落射荧光二向色镜分束器仅针对LED或类似光引擎等宽带光源进行了优化。
BrightLine二向色分束器，用于DAPI/FITC/TRITC/Cy5/Cy7成像通道，针对Lumencor CELESTA光引擎进行了优化，使用408 nm、473 nm、545 nm、635 nm和750 nm波长。
| Optical Specifications | Value |
|---|---|
| Transmission Band 1 | Tavg > 93% 426 – 456 nm |
| Transmission Band 2 | Tavg > 93% 498 – 524 nm |
| Transmission Band 3 | Tavg > 93% 574 – 611 nm |
| Transmission Band 4 | Tavg > 93% 667 – 701 nm |
| Transmission Band 5 | Tavg > 93% 784 – 850 nm |
| Reflection Band 1 | Ravg > 95% 369 – 413 nm |
| Reflection Band 2 | Ravg > 95% 471 – 483 nm |
| Reflection Band 3 | Ravg > 95% 541 – 557 nm |
| Reflection Band 4 | Ravg > 95% 630 – 647 nm |
| Reflection Band 5 | Ravg > 95% 725 – 757 nm |
| Edge Wavelength 1 | 421 nm |
| Edge Wavelength 2 | 491 nm |
| Edge Wavelength 3 | 567 nm |
| Edge Wavelength 4 | 659 nm |
| Edge Wavelength 5 | 776 nm |
| General Filter Specifications | Value |
| Angle of Incidence | 45 ± 1.5 degrees (Ravg > 95% & Tavg > 90%), 45 degrees (Tavg > 93%) |
| Cone Half-angle | 2 degrees |
| Optical Damage Rating | Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours. |
| Reflected Wavefront Error | >> 6λ P-V RWE @ 632.8 nm |
| Steepness | Standard |
| Flatness / RWE Classification | Standard Epi-fluorescence |
| Physical Filter Specifications | Value |
| Transverse Dimensions (L x W) | 25.2 mm x 35.6 mm |
| Transverse Tolerance | ± 0.1 mm |
| Clear Aperture | ≥ 80% (elliptical) |
| Scratch-Dig | 60-40 |
| Filter Thickness (unmounted) | 1.05 mm |
| Filter Thickness (unmounted) | 1.05 mm |
| Filter Thickness Tolerance (unmounted) | ± 0.05 mm |
| Filter Thickness Tolerance (unmounted) | ± 0.05 mm |
| Orientation | Reflective surface marked with part number – Orient in direction of incoming light |
